Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.In order to evaluate the possibility of using the epibenthic marine amphipod Hyale nigra
in toxicity tests, a sensitivity test using spiked sediment method was applied, with cadmium chloride
as a reference toxicant (CdCl2). Tests with environmental samples were performed to validate the test
organisms. The samples were taken from Santos Estuary and Bay in state of Sao Paulo, Brazil. The
historical toxicity and contamination are mentioned in the literature. The results obtained in the spiked
sediment tests showed little variation in the sensitivity of Hyale nigra amphipod with a mean LC50 value
of 5.73 mg.L-1 (1.51 mg.L-1 – 8.75 mg.L-1). In the tests with environmental samples, the results obtained
were similar to those found in the literature regarding this sampling area. This study demonstrates the
potential of Hyale nigra amphipod as a test organism in toxicity tests, once that the specie is sensitive and
its results were similar to those reported in the literature on other species of amphipods that are already
used in ecotoxicological studies.
Keywords: Sediment; Amphipod; Hyale nigra; Spiked sediment.
Published on: Nov 18, 2017 Pages: 25-30
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DOI: 10.17352/alo.000006
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